RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CLONAZEPAM AND PAROXETINE IN TABLET DOSAGE FORMS

*Srikanth A.*, Shaik Mohammed Yusuf, S. Siva Prasad, Afroz Begum S., Sivakala T., K. Yalla Reddy

ABSTRACT

A simple accurate, precise rapid isocratic RP-HPLC method development for the simultaneous estimation of Paroxetine and Clonazepam in tablet dosage forms. The chromatographic system was carried on INERTSIL (250x4.6mm, 5μ) column using mobile phase of phosphate buffer: acetonitrile: methanol in the ratio of 30:30:40 v/v at a flow rate of 1.0 ml/min. the eluents was detected at 268nm. The retention time of paroxetine was found to be 4.867 min and retention time for clonazepam was found to be 2.367 calibration curve was linear over the concentration range of paroxetine is 60-140 μg/ml and concentration range of clonazepam is 2.4-5.6 μg/ml the correlation coefficient for both peak was found to be 0.999 and 0.998 respectively. All the analytical validation parameters were determined and found in the limit as per ICH guidelines.

Keywords: Paroxetine, Clonazepam, RP-HPLC, Validation, Phosphate buffer.