ISOLATION, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF FATTY ACID METHYL ESTERS (FAMEs) FROM VINCA ROSEA SEEDS

Meshram Gangadhar*, Kulkarni Tanushri, Khamkar Shraddha and Metangale Ganesh

ABSTRACT

Vinca rosea is a perennial shrub of the Apocynacae family used as an ornamental shrub as well as medicinal herb. One of the therapeutic approaches for preventing diabetes mellitus type 2 is to retard the absorption of glucose via inhibition of α-glucosidases viz. glucoamylase and α-amylase. In the present study, fatty acids methyl esters (FAMEs) are isolated from the seeds of Vinca rosea ethanol extract. Their structures were elucidated on the basis of GC-MS data, as methyl stereate, methyl oleate and methyl palmitate. FAMEs exhibited potent inhibitory activity against glucoamylase and α- amylase with IC50 value 67.98 μg/mL and 73.89 μg/mL respectively. The antioxidant activity of FAMEs was evaluated by DPPH method. The results indicate that FAMEs possesses considerable antioxidant activity. The highest radical activity was detected (IC50=137.30 μg/mL). This investigation systematically supports the use of FAMEs to study the role of antioxidant therapy in the management of Diabetes Mellitus Type 2.

Keywords: Vinca rosea, FAMEs, glucoamylase, ?-amylase, antioxidant activity.