HEPATOPROTECTIVE AND ANTIOXIDANT EFFECT OF ETHANOLIC AND PURIFIED FLAVONOID EXTRACTED FROM C. ROTUNDUS AGAINST CARBON TETRACHLORIDE INDUCED LIVER DAMAGE IN MICE (IN VIVO)

Essam F. Al-Jumaily* and Jameelah K.T. Al-Isawi

ABSTRACT

In this study an interaction was carried out between one dose of CCl4 (3.2 mg/Kg) and the two doses (150 and 300 mg/kg) of ethanolic and purified flavonoid extract C. rotundus and one dose of vitamin C (180 mg/kg) were evaluated in mice (in vivo) given injection for 14 days inducting biochemical function of three antioxidant enzymes (Catalase, Superoxide dismutase and Glutathione) in liver homogenate, liver function enzymes and histopathological changes of liver. The results showed that the antioxidants glutathione (GSH) and Superoxide dismutase (SOD) and catalase (CAT) has increased significantly (P<0.05t) after the animals has been injection mice CCl4, and that each of the purified flavonoids and ethanolic extract at a concentration (150 and 300 mg / kg) has led to decreased Glutathione enzyme (GSH) activity significantly (P <0.05)in compared to the ct . The ethanolic extract for C. rotundus concentration of 150 mg / kg and vitamin C concentration of 180 mg / kg has increased significantly (P <0.05) when compared with the treatment of control group. Either Superoxide dismutase (SOD) has led purified flavonoids from C. rotundus (150 and 300 mg / kg) to increase significantly at (P <0.05) level compared to the treatment of tetrachloride carbon an which the enzyme declines significantly at (P < 0.05) level, when injection them for 14 days. Either enzyme catalase (CAT) has led purified flavonoids extract and ethanolic extract of C. rotundus (150 and 300 mg / kg) to decrease significantly (P <0.05) compared to treatment tetrachloride carbon. Examination of liver tissue confirmed potential histopathological treatment with CCl4 and purified flavonoid extract shows necrosis of hepatocyte cells with inflammatory cells infiltration. Furthermore, the liver of animal that treatment with CCl4 and ethanolic extract shows adepletion of glycoprotein granules with dispersed necrosis of hepatocyte cells.

Keywords: ethanolic, rotundus, histopathological, ethanolic.