RP-HPLC METOD DEVELOPMENT AND VALIDATION ON TIAGABINE IN BULK AND PHARMACEUTICAL DOSAGE FORM
Sapavath Radha* and Dr. M. Dhanalakshmi
ABSTRACT
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the
validated of Tiagabine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a
Phenomenex Gemini C18 (4.6×250mm) 5μ column using a mixture of Methanol and Water (50:50 v/v) as the
mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 247nm. The retention time of the
Tiagabine was 2.187 ±0.02min. The method produce linear responses in the concentration range of 30-150ppm of
Tiagabine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the
quality control of bulk and pharmaceutical formulations.
Keywords: Tiagabine, RP-HPLC, validation.
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