EFFECT OF POLYHERBAL FORMULATION ON NEUTROPHIL FUNCTION AND TDAR IN WISTAR ALBINO RAT

Isah Suleiman Yahaya, Mohammed Haruna Yeldu, Kabir Magaji Hamid*, Usman Musa and Mustapha Umar Kalgo

ABSTRACT

This study evaluate the effect of polyherbal formulation (PHF) on Neutrophil Function and T-cell dependent antibody response in Wistar Rat. The result shows that there is significance difference in Neutrophil Adhesion (NA) test across the seven groups (p<0.001). When comparing between group 6 (treated with PHF) and 7 (treated with polyherbal formulation and cyclophosphamide) there is significance difference in NA (p=0.009). Nitroblue tetrazolium (NBT) reduction test result shows that there is significance difference across the groups (p < 0.001). There is significant difference in NBT reduction between group 1 (normal control) and group 6 (PHF highest dose), group 2 (Positive control) and group 6, group 6 and group 7 (p=0.009), and group 3 (Negative control) and group 7 (p=0.01), however there is no significance difference between group 1 compared with group 7, (p=0.033). In T-cell dependent antibody response the study revealed that there is significance difference in concentration of both KLH IgM and IgG between the seven groups (p<0.001). When comparing group 1 and 6, group 3 and 7 as well as group 6 and 7 there is significance difference in concentration of KLH IgM (p = 0.009). Similarly there is significance difference in comparing group 1 and 6, 2 and 6, 3 and 7 as well as 6 and 7 on KLH IgG concentration (p = 0.009). Neutrophil function and serum concentration of IgM and IgG against KLH increase with increase dose of PHF up to 1000 mg/kg/bwt. The PHF has potential immunomodulatory activity in wistar rat.

Keywords: Immunomodulation, Medicinal, Neutrophils, Plants, T-Lymphocytes.