THE EFFECT OF 5 FRACTIONAL EXTRACTS OF ACER TEGMENTOSUM MAXIM. CONCENTRATED IN DECOMPRESSED VACUUM ON DENTAL CARIES-INDUCING BACTERIA

Hae-Gyung Yoon, Gil-Hyun Lee and Kyung-Yae Hyun*

ABSTRACT

Acer tegmentosum Maxim. (Acer TM) was concentrated in decompressed vacuum to obtain 5 factional extracts, and the amount of dental caries-inducing bacteria and periodontitis-inducing bacteria that were inhibited depending on the concentration of Acer TM was examined. The concentration of proteins contained in Acer TM Fractions 1, 2, 3, 4 and 5 was 200 (mg/mL), 225 (mg/mL), 110 (mg/mL), 150 (mg/mL) and 90 (mg/mL) respectively. The protein concentration of Acer TM Fraction 2 was the highest. The antibacterial effect of each Acer TM fraction on S. mutans was examined, and no antibacterial effect was observed in Fractions 1, 2 and 3. In the extract of Fraction 4, 2mm and 4mm antibacterial zones were observed at 50mg/ml and 100mg/ml respectively. In the extract of Fraction 5, 2mm and 4mm antibacterial zones were observed at 50mg/ml and 100mg/ml respectively. In the case of P. gingivalis, no antibacterial effect was observed in Fractions 1, 2 and 3. In Fraction 4, 1mm, 3mm, 6mm and 8mm antibacterial zones were observed at 10mg/ml, 25mg/ml, 50mg/ml and 100mg/ml respectively. In the extract of Fraction 4, an antibacterial effect was observed across the entire concentration range from the low concentration to the high concentration. In the extract of Fraction 5, 4mm and 10mm antibacterial zones were observed at 50mg/ml and 100mg/ml respectively, showing the highest antibacterial effect. Therefore, the extract of Acer TM Fractions 4 and 5 seems to be used as a candidate natural plant to treat periodontal diseases that induce dental caries-causing bacteria.

Keywords: Acer tegmentosum Maxim., fractional extract, S. mutans, P. gingivalis, antibacterial effect.