FIXATION & VARIOUS FIXATIVES USED AS AN ALTERNATIVE TO FORMALIN-A REVIEW

*Dr. Neha Vaid. Deepak Bhargava, Rajeshwar Chawla, Deepak Goyal, Ruby Bansal, Chander Udey Singh Pawar

ABSTRACT

Fixation is usually the first step to prepare biological specimens for microscopy. Any treatment which will preserve cell structure and its biochemical composition can be deemed to be fixation. Of course, the quality of fixation is the key for all following steps which are necessary in histological research. Hence, preservation of cells with minimal alteration of morphology and virtually no loss of molecules is essential in tissue preservation. The purpose of the present article is to provide adequate insight about the fixation and various fixatives used as an alternative to formalin.

Keywords: Fixation, fixatives, biopsy.