ANTIMICROBIAL ACTIVITY OF OCTAPEPTIDES RELATED TO HUMAN HISTATIN 8

Pandurangan Perumal*, Vijay Prakash Pandey and Prabu Seenivasan

ABSTRACT

Objective: To Synthesize, characterize and antimicrobial evaluation of octapeptides related to human histatin 8. Method: Solid phase peptide synthesis (SPPS) by Fluorenyl methyl oxycarbonyl (Fmoc) chemistry protocol. Results: The N-terminal portion of HH-1, HH-2 and HH-3 showed high potential against gram negative bacteria. The peptides were evaluated for antimycobacterial activity against Mycobacterium tuberculosis H37 RV and Clinical isolates of M. tuberculosis resistant strain by Luciferase Reporter Phage assay (LRP). Among the tested peptides, the peptide HH3 has been showed maximum percentage of reduction in relative light units (RLU) against Mycobacterium tuberculosis- H37 Rv and clinical isolate of M. Tuberculosis resistant at the concentration of 100 μg/mL. Conclusion: octapeptides related to human histatin 8 were synthesized, characterized and exhibited promising antibacterial, antifungal and antimycobacterial activity.

Keywords: Antimicrobial peptides; Solid Phase Peptide Synthesis; Human histatin 8; Luciferase Reporter Phage assay; Mycobacterium tuberculosis.