DRIED POWDER OF UNANI AND AYURVEDIC MEDICINES YIELDS REMARKABLE DNA UPON EXTRACTION

Nishat Chowdhury*, Shingo Ueno, Md. Forhad Hasan, Md. Saiful Hoque, Kazi Linkon and Lubna Akter

ABSTRACT

Genomic DNA extraction is an important aspect of plant molecular biological research. The objective of the study was to recommend the cheap and efficient genomic DNA extraction method for some kobiraki medicines in Bangladesh. The modified plant genomic DNA extraction methods extracted drupe, pome, hespiridium, pepo and complex fruits. Extraction kit (Qiagen) method were applied with eight different ayurvedic medicine powder such as Phyllanthus emblica, Withania somnifera, Abroma augusta, Syzygium cumini, Oroxylum indicum, Chrysogenum aurium, Mucuna pruriens, Terminalia bellirica. Based on the quantity of the extracted genomic DNA tested by measuring the absorbance at 260 nm using Nanodrop® ND-1000 spectrophotometer, quality determined by the ratio of A260 / A280 and the amplifiable quality of DNA determined by the horizontal agarose gel electrophoresis using 1% agarose in TBE buffer at constant voltage of 60V, the method explained by Cheng et al and the Genomic DNA extraction kit yielded good quality DNA with satisfactory concentration for all the powder medicine tested. Therefore the modified method of Cheng et al, 1987 could be recommended for the efficient and cost effective DNA extraction from fruit species instead of the commercially available expensive and chemically hazardous DNA easy plant kit method.

Keywords: Phyllanthus emblica, Withania somnifera, Abroma augusta, Syzygium cumini.