EVALUATE NEUROPROTECTIVE EFFECT OF HESPERIDIN USING CHRONIC UNPREDICTABLE STRESS MODEL

G. Devala Rao, Hanumanthu Penchalaiah* and S. Praveen Begum

ABSTRACT

Current study was investigate the effect of hesperidin on unpredictable chronic stress induce behavioral and biochemical alterations in mice. Chronic unpredictable stressors can produce a situation similar to clinical depression, and such animal models can be used for the preclinical evaluation of antidepressants. Many findings have shown that the levels of proinflammatory cytokines (e.g., TNF-α) and oxidative stress (increased lipid peroxidation, decreased glutathione levels, and endogenous antioxidant enzyme activities) are increased in patients with depression. Mice were subjected to different stress paradigms daily for a period of 45 days to induce depressive like behavior such as memory acquisition, and retention. Chronic treatment with hesperidin significantly reversed the unpredictable chronic stress-induced behavioral (improve memory function), biochemical changes (decreased glutathione levels, superoxide dismutase), and inflammation surge (serum TNF-α IL 1β) in stressed mice. The study revealed that hesperidin exerted effects in behavioral despair paradigm in chronically stressed mice, specifically by modulating central oxidative stress and inflammation.

Keywords: Hesperidin; TNF-?; IL 1 ?; Unpredictable chronic stress; Antioxidants.