IN VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF CITRUS LIMON LINN. (LEAVES)

Vijeta Gupta*, Dr. Vijender Singh and Dr. Mojahidul Islam

ABSTRACT

Citrus limon linn belonging to Rutaceae family is highly reputed plant and has been widely employed in herbal medicine and aromatherapy but no work has been carried out on the antioxidant activity of the leaves extracts. The antioxidant property of methanolic extract of Citrus limon Linn. was evaluated by estimation of Total Flavonoid content (TFC), Free radical scavenging activity (DPPH- radical scavenging activity) and Total Phenolic content (TPC). The Total flavonoid content was measured by Aluminum chloride colorimetric method at 420 nm and was expressed as mg quercetein equivalent (QE)/gm dry plant material. The DPPH radical scavenging activity of C.limon Linn was estimated by Inhibitory concentration (IC50) at 517 nm and the Total Phenolic content was analyzed by Folin - ciocalteu colorimetric method using gallic acid as standard (GAE), absorbance was reported at 765 nm. Results were reported as Total Flavonoid content was 3.73 mg/gm QE (quercetin equivalent), DPPH radical scavenging activity (IC50 value) was 310 μg/ml and Total Phenolic content was 1.96 mg/gm GAE (gallic acid equivalent). Thus from ongoing study it is concluded that leaves of Citrus limon Linn. possess significant Antioxidant activity and can be used for further research study.

Keywords: Anti oxidant activity, Citrus limon Linn.