ANALYSIS OF ORAL MICROORGANISMS BEFORE AND AFTER PERIODONTAL TREATMENT USING REAL-TIME PCR

Yun-Kyung Kim* and Gey-Pyo Kim

ABSTRACT

It is important to analyze oral microorganisms in patients with periodontal disease because this disease is caused by the proliferation of specific types and numbers of microorganisms in the oral cavity. The aim of this study was to analyze the changes in the number and type of pathogenic microorganisms in the oral cavity using real-time PCR before or after periodontal treatment in patients aged 50 years or more who visited the D dental clinic in Busan. The levels of pathogenic microorganisms before and after periodontal treatment were 12.44 ± 1.76% and 7.01 ± 0.10%, respectively. Thus, the level after periodontal treatment was significantly decreased compared to before treatment. Analysis of the major pathogens of dental caries revealed that Sm, Ss, and major pathogens of periodontal disease, including Ac, Pg, Tf, Td, Pi, Fn, Pm, Cr, Pn, and En, were significantly decreased compared to before periodontal treatment. Quantitative analysis of periodontal pathogens in the clinical by real-time PCR demonstrated the effectiveness of the treatment, which will help to improve the oral hygiene management ability of patients.

Keywords: Dental clinic, Oral microorganisms, Periodontal disease, Periodontal treatment, Real-time PCR.