EXTRACTION, PHYTOCHEMICAL EVALUATION AND CHARACTERIZATION OF POLYPHENOLS AND FLAVONOIDS FROM AQUEOUS METHANOLIC LEAF EXTRACTS OF INDIGENOUS PLANT CLERODENDRUM COLEBROOKIANUM WALP AND CENTELLA ASIATICA LINN OF NORTH EAST INDIA

Ratna Jyoti Das*, Daphisha Marbaniang and Bhaskar Mazumder

ABSTRACT

Extraction of polyphenols and flavanoids were done for two indigenous plants Clerodendrum colebrookianum walp and Centella asiatica. Extraction was done by both cold maceration and continuous hot percolation. Determination of total phenolic as well as and flavanoid content was carried out. Characterizations was done by TLC study, UV analysis, melting point determination and FTIR study. More total phenolic content found in Clerodendrum colebrookianum walp (621.02 ± 0.03 mg of GAE/gm plant extract) and content of total flavonoid was was found more in Centella asiatica L.(596.40 ± 0.53 mg of Quercetin equivalent/gm plant extract). UV and FTIR study concluded the existence of both flavonoids and phenolics in the respective extracts as compared with gallic acid and quercetin. Quantity of phenolic as well as flavanoid exhibit the indication of antioxidant action of the particular plants. Both the plants showing rich in total polyphenols and total flavonoids. Datas were mean ± SD moreover analyzed by means of One-way ANOVA, via Graphpad INSTAT. Confidence interval has been measured as 99% and p< 0.01 were considered significant. Phenolics encompass to possess an imperative antioxidant activity headed for free radicals, which is predominantly based on the redox properties of their phenolic hydroxyl groups and the structural associations among different parts of their chemical structure. It has been customary a highly positive rapport between total phenols along with antioxidant action in loads of plant species.

Keywords: Polyphenols, flavonoids, extraction, indigenous plant, phytochemical evaluation.