ASSOCIATION OF XRCC1 399GLN AND XRCC3 T241 METH POLYMORPHISM WITH THE RISK OF CERVICAL CANCER IN NORTH INDIAN POPULATION

Mark Rector Charles, M.Sc, Syed Tasleem Raza*, Ph.D, Pushpendra Pratap, M.Sc, Rolee Sharma, Ph.D, Jamal Mohd. Arif, Ph.D and Ale Eba, M.Sc

ABSTRACT

Association of XRCC1 399Gln and XRCC3 T241 Meth polymorphism with the risk of cervical cancer in North Indian Population. Objective: To study the association of polymorphisms in the DNA repair genes of x-ray repair cross-complementing (XRCC) gene 1 399Gln and XRCC3 T241M, to the susceptibility of cervical cancer (CC) in North Indian population. Material and Methods: XRCC1 & XRCC3 polymorphisms were characterised in Seventy-one eligible CC patients and Sixty nine healthy controls who had no history of any malignancy by using confronting two pair primers (PCR–CTPP) method, taking DNA from peripheral blood in a case control study. Results: Our results revealed that the frequencies of AA genotype of XRCC1 399 polymorphism were significantly higher in the CC patients than in the normal individuals (p 0.001), OR=5.27, 95% CI= (1.934-14.372). While we did not observe any association between the XRCC3 T241M polymorphism and CC risk. Conclusion: Our results suggested that, XRCC1 399 gene is an important candidate gene for susceptibility to cervical cancer. Although the sample size was small, the present study indicate a statistical association between cervical cancer and XRCC1 399Gln polymorphism. Future studies are needed that may provide a better understanding of the association between gene polymorphism and cervical carcinoma risk.

Keywords: XRCC1, XRCC3, Gene polymorphism, Cervical cancer.