ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL EVALUATION OF TRIBULUS TERRESTRIS FRUIT

Pallavi Duse*, Tanvi Pingale*, Vipul Duse and Dr. Arti Jagtap

ABSTRACT

Reactive oxygen species (ROS) are thought to underline the process of ageing and the pathogenicity of various diseases, such as cardiovascular diseases. The use of traditional medicine is widespread and plants still present a large source of natural antioxidants that might serve as leads for the development of novel drugs. The purpose of our study is to investigate the antioxidant activity and phytochemical evaluation of Tribulus terrestris fruit. (Tanvi Pingale and Kedar Prabhavalkar, 2016). In the present study, DPPH assay was used to estimate the antioxidant activity of ATT and it showed IC 50 at 400μg/ml. It suggests that ATT has good free radical scavenging activity, as lower the IC 50 value higher is the free radical scavenging activity, another method used for the estimation of ATT antioxidant potential was total reducing capacity. This method is based on the conversion of ferric (Fe 3+) – ferricyanide complex to ferrous (Fe 2+) form by ATT. Higher the total reducing capacity greater is the antioxidant activity of plant extract. (Yerra Rajeshwar et. al 2005). ATT has shown good total reducing capacity.

Keywords: Tribulus terrestris, DPPH, HPTLC fingerprint, Zygophillaceae, ferricyanide.