DETERMINATION OF NARINGENIN IN HYDROALCOHOLIC EXTRACT OF COMMIPHORA MUKUL (BURSERACEAE) BY HPTLC METHOD

Akshay D. Dhamane and Padmanabh B. Deshpande*

ABSTRACT

Naringenin is a naturally occurring flavanone (flavonoid) identified to have a bioactive effect on human health and is mainly found Commiphora mukul as one of the active constituent. Naringenin possesses various biological activities such as antidiabetic, antiatherogenic, antidepressant, immunomodulatory, antitumor, anti-inflammatory, DNA protective, hypolipidaemic, antioxidant, peroxisome proliferator-activated receptors (PPARs) activator, and memory improving. In the present study, an attempt was made to quantify the flavonoid Naringenin in the Commiphora mukul hydroalcoholic extract. TLC was performed to confirm the presence of Naringenin and HPTLC procedure has been developed for quantification of Naringenin in the hydroalcoholic extract. HPTLC separation was achieved on Merck TLC aluminium sheets precoated with silica gel 60F254 using Toluene: Ethyl acetate: Formic acid (6: 4: 1, v/v/v) as mobile phase. Quantitative analysis was carried out at 289 nm. A good linear relationship was obtained for Naringenin in the concentration range of 200-1200 ng band-1.

Keywords: Commiphora mukul, Flavonoid, Naringenin, HPTLC.