EVALUATION OF ALCOHOLIC EXTRACTS OF SESAMUM INDICUM L. SEEDS COLLECTED FROM PUNJAB REGION OF PAKISTAN ON WOUND HEALING AND SKIN REGENERATION IN RATS.

Sheraz Khan*, Saniya Hashim Khan, Taimur Ali and Narmeen Hashim

ABSTRACT

The aim of this study is to confirm and compare the wound healing effects of sesame oil on experimentally induced excision wound, incision wound, and burn wound models in rats. Sesame oil obtained from different methods of extraction was applied on induced excision wound, incision wound and burn wound models in 5 groups of Sprague-Dawly rats. Each group had 6 rats. Group I-III were topically treated with 2ml of various and pure extracts of sesame oil. Group IV was treated with the standard treatment of Aloe vera extract (10% gel formulation) while Group V received no treatment for 25 days. In incision wound model, ANOVA and Dunnett's test were applied (P < 0.05) to compare relative wound area. For excision and burn wound models, the wound contraction-50% (days) was determined by plotting the wound area vs days on a graph paper. Comparatively, noticeable decrease in wound length and increased epithelialization period was observed in incision wound model and better rate of closure was found in sesame oil groups (P < 0.05). In the burn and excision wound models, significant reduction (P<0.001) in wound contraction (50%) and epithelialization period was also noted in sesame oil groups. Overall, an improved healing pattern was found in rats who received various extracts of sesame oil corresponding to standard treatment while sesame oil extract of ethyl acetate was found to have highest potential for wound healing than other extracts. The findings showed that sesame oil extract of ethyl acetate has much better potential for wound healing and skin regeneration in rats.

Keywords: Excision, Incision, Burns, Sesamum indicum, Wound healing, Epithelialization.