AUGMENTED POINTS OF LIPID PEROXIDATION AND APPEARANCE OF PROPHETIC VARIABLES AND THEIR AFFILIATION TO DEVELOP RHEUMATOID ARTHRITIS

Muhammad Waqas*, Umar Saeed, Saman Saeed, Shoaib Asif, Arif Malik and Rabbia Tariq

ABSTRACT

Objective: Assessment of upstream levels of lipid peroxidation and DNA damage regulating the development of rheumatoid arthritis. Study design: Cross sectional comparative study. Place and duration of study: The study was carried out in the institute of molecular biology and biotechnology (IMBB) in The University of Lahore-Pakistan since last two years. Material and methods: Blood, saliva and synovial fluid samples of 75 individuals diagnosed with Rheumatoid Arthritis were collected from Jinnah Hospital Lahore while the samples of 75 normal individual were served as a control. Levels of MDA were determined spectrophotometrically while the concentrations of isoprostanes, 8-OHdG and HNE were measured from commercially available Elisa Kit. Results: Significantly high level of oxidative stress markers were recorded in serum, saliva and synovial fluid of rheumatoid arthritis patients as compared to control. Levels of lipid peroxidation products including MDA (μmol/ml), isoprostanes(pg/ml) and HNE (μmol/ml) and DNA damage in the form of 8-OHdG (pg/ml) were recorded significantly high (p<0.015, p<0.022, p<0.004 and p<0.036 respectively) in rheumatoid Arthritis patients as relative to the normal individuals. Levels of MDA in serum (1.95±0.094/0.95±0.019), saliva (0.012±0.0034/0.056±0.0056) and synovial fluid (3.26±0.65/0.019±0.0016) were differed significantly in both groups. Level of isoprostanes in serum (12.26±5.26/1.26±0.015), saliva (2.16±0.019/0.816±0.017) and synovial fluid (34.26±4.26/0.136±0.019) were recorded higher in RA patients as compared to control. Concentrations of 8-OHdG in serum (0.945±0.014/0.019±0.0035) saliva (0.0024±0.0003/0.0029±0.00017) and synovial fluid (1.33±0.451/0.055±0.0016) were recorded high in RA patients. Significantly high concentration of HNE in serum (4.265±1.25/1.99±0.016), saliva (1.26±0.15/0.191±0.0091) and synovial fluid (6.35±1.16/0.094±0.00165) were recorded in RA diagnosed group relative to control. Conclusion: Oxidative stress biomarkers including lipid peroxidation products contribute to the onset of inflammation and autoimmunity in RA patients.

Keywords: Oxidative Stress, RA, lipid peroxidation, MDA, isoprostane, 8-OHdG, HNE.