EVALUATION OF MIXTURE OF ETHANOL, SALT AND PALM OIL AS HOME REMEDY FOR THE RELIEF OF SYMPTOMS ASSOCIATED WITH SICKLE CELL

Ademokoya A. A.*, Ajayi A. O. and Akinyosoye F. A.

ABSTRACT

Aim: Sickle cell disease has been a global medical concern from time immemorial and the only panacea for the treatment of the disease is the bone marrow transplantation method that requires huge amount of dollars to achieve. Therefore the evaluation of mixture of ethanol, salt and palm oil in water used locally and proved effective in Akungba Akoko in Ondo State as a home remedy for the relief of symptoms associated with sickle cell disease was evaluated in this study. Methodology: The mixture was compounded from domestically produced palm oil, table salt, while the ethanol was purchased from licensed shop in Akugba-Akoko, Ondo State. Microbial decontamination of the mixture was carried out using standard microbiology techniques. The acute and sub-acute toxicological assessment of the mixture was carried out haematologically, biochemically, and histopathological using standard animal inoculation assays. The spectrum peak pick report was done using UV-1800 series. Result: Acute toxicity results showed no LD50 since there was no mortality among the experimental animals at the dosage or concentration applied. There was no significant (P<0.05) increased in the level of serum transaminase, proteins and High Density Lipoprotein (HDL) cholesterol, likewise, the mixture has no significant reduction (P>0.05) in the level of leucocytes as well as neutrophils, basophils and monocytes in both animal’s genders. Moreover, there was no variation in serum triglycerides as well as liver, spleen, and testiest proteins. The histopathology of the rat’s organs showed no inflammation or vascular congestion of hepatocytes. In the rat’s lung and liver, no infiltration of the leucocytes in peri-portal veins was noticed. Concerning the study data, One-way ANOVA was done using Statistical Package for Social Science (SPSS) software version 20. Significant differences between control and the experimental rat groups were assessed by Waller Duncan test. Conclusion: The result of this study shows that oral administration of sickle cells home remedy mixture is not associated with any signs of toxicity in the organs studied. Therefore, approval from a government agency should be sought so as to produce and make available for the sufferers of the disease.

Keywords: Toxicological, evaluation, sickle cell, home remedy, mixture.