VALIDATED RP - HPLC METHOD FOR THE DETERMINATION OF FEBUXOSTAT IN BULK AND PHARMACEUTICAL DOSAGE FORM

Chusena Narasimharaju Bhimanadhuni*, Devala Rao Garikapati and Erla Nagachandrika

ABSTRACT

A reverse phase high performance liquid chromatographic method was developed for the determination of Febuxostat in bulk and Pharmaceutical dosage form. The separation was effected on a Zodiac C18 column (250 mm x 4.6 mm; 5μ) using a mobile phase mixture buffer of 0.01M potassium dihydrogen phosphate and acetonitrile (50:50 v/v) and the PH was adjusted to 6.5 with orthophosphoric acid at a flow rate of 1ml/min. The detection was made at 315 nm. Calibration curve was linear over the concentration range of 20-120 μg/ml of Febuxostat. The propose method was validated as per the ICH guidelines. The method was accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and Pharmaceutical dosage form.

Keywords: Buffer, Acetonitrile, Febuxostat, Tablets, Zodiac C18 column, RP-HPLC.