IMPACT OF ETHANOLIC EXTRACT OF SENNA AURICULATA, PHYLLANTHUS EMBLICA.L., SYZYGIUM CUMINI(L.) SKEELS USED BY RURAL AREAS OF RAJASTHAN STATE, INDIA ON DIABETIC NEUROPATHY IN RATS.

Ashish Kumar Sharma* and Charanjeet Singh

ABSTRACT

Purpose of the research: India is ―diabetes capital of the world‖. Rise to 69.9 million by 2025; major causes of morbidity and mortality. Herbal formulations are preferred to minimize the risk and low cost, traditionally. Metohods: Alloxan(150mg/kg)i.p. results in diabetic rats given Senna auriculata leaf, Phyllanthus emblica.L. fruits and Syzygium cumini(L.) Skeels seeds(150 mg/kg)p.o. extracts, for 42 days. Parameters of diabetic neuropathy and histopathology was done. The Principal Results: In Diabetics, Blood Glucose level(BGL)(84.42±6.384 to 369.36±7.784mg/dl); Muscle Grip Strength(MGS)(59.32±1.052 to 13.52±0.883seconds); thermal pain response(TPR) (5.55±0.621 to 13.67±1.164seconds). blood protein(7.48±0.051 to 25.18±0.046mg/dl); urine protein(0.692±0.061 to 2.68±0.056mg/dl); blood albumin(1.94±0.043 to 0.248±0.007mg/dl); urine albumin(0.082±0.009 to 2.68±0.056mg/dl);. Ethanolic extract of Senna auriculata leaf, Phyllanthus emblica.L. fruits and Syzygium cumini(L.)Skeels seeds & combination treated groups found BGL124.42±7.042, 112.07±6.942, 126.25±7.051 & 98.83±6.932mg/dl; MGS49.06±0.962, 52.05±1.247, 54.06±1.268 &56.79±1.125seconds; TPR6.54±0.841, 7.38±0.802, 6.45±1.062 & 6.14±0.837seconds; blood protein7.98±0.039, 8.02±0.053, 8.06±0.039 & 7.48±0.045mg/dl; urine protein1.22±0.058, 0.94±0.049, 0.96±0.056 & 0.82±0.062mg/dl; blood albumin1.64±0.033, 1.82±0.036, 1.87±0.044 & 1.96±0.039mg/dl; urine albumin0.122±0.008, 0.098±0.007, 0.132±0.009 & 0.108±0.011mg/dl. Conclusions: Combination of Senna auriculata leaf, Phyllanthus emblica.L. fruits and Syzygium cumini(L.) Skeels seeds (150 mg/kg) p.o. extracts normalizes biochemical parameters & Morphological changes sciatic nerve and improves behavioral parameters, effective in treatment of diabetic neuropathy.

Keywords: Senna auriculata leaf; Phyllanthus emblica.L. fruits; Syzygium cumini(L.)Skeels seeds; diabetic neuropathy.