EVALUATION OF ANTI-MICROBIAL ACTIVITIES OF POLYHERBAL SIDDHA FORMULATION PANJAMUSTI CONJEE AGAINST HUMAN RESPIRATORY PATHOGENS

Vaniswari D. S.*, Sivaranjani K. and Shyamala K.

ABSTRACT

Aim: The aim of the study is to screen In-vitro Anti-microbial activities of aqueous extracts of Panjamusti conjee-A Siddha formulation. Methodology: Panjamusti conjee was prepared as per Siddha Materia Medica procedures. Aqueous extract of test sample-Panjamusti conjee was prepared by Kirbauy method. In-vitro anti microbial activity of extract of test sample was screened against human respiratory pathogenic micro organism namely Staphylococcus aureus and Streptococcus pneumoniae using disc diffusion method. The micro organisms were collected from the Malar Micro Diagnostic Centre, Palayamkottai, Tamilnadu, India. Sterilized discs were soaked in aqueous extract of test sample individually at the concentration of 20mg/disc. Anti-bacterial suspension was inoculated in Muller –Hinton Agar Media. Amikacin was used as standard drug for the Antimicrobial study. Zone of inhibition was measured and recorded. Result: Aqueous extract of test sample Panjamusti conjee showed more anti-bacterial activity against human pathogenic micro organism namely Staphylococcus aureus(18 mm) and Streptococcus pneumoniae (20 mm). Conclusion: It is concluded that the test sample Panjamusti conjee can be prescribed as the medicine for diseases caused by pathogenic Staphylococcus aureus and Streptococcus pneumoniae in the respiratory tract.

Keywords: Siddha herbal, Panjamusti conjee, Antimicrobial sensitivity testing.