DPPH AND HYDROXYL RADICAL SCAVENGING ACTIVITY EFFECT OF SEDUM SARMENTOSUM

In Sook Kye and Man Kyu Huh*

ABSTRACT

Antioxidants play an important role in human health protection. The antioxidant properties of 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and hydroxyl radical (OH) scavenging activity derived from water, ethanol extracts of Sedum sarmentosum were evaluated. DPPH scavenging activity was analyzed according to the method of Brand-Williams et al. Hydroxyl radical (OH) scavenging activity was measured by the Fenton reaction. DPPH scavenging activity of distilled water extract (DWE) with raw leaves evaluated was 37.1% at 1.0 mg/ml and that of ethanol extract (ETE) was 38.7% at same concentration. The all values of DPPH scavenging activity of raw extracts of S. sarmentosums were higher than those of boiled extracts. OH scavenging activity of raw leaves of S. sarmentosum was evaluated at 1.0 mg/ml of water extracts was 22.5%, that of stems was 8.3% at same concentration. The boiled extract values of OH scavenging activity of S. sarmentosum were lower than those of raw extracts. The all groups showed a statistically significant difference (p>0.05).

Keywords: 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), hydroxyl radical (OH), Sedum sarmentosum.