BIOANALYTICAL METHOD DEVELOPMENT IN HUMAN PLASMA BY USING LC-MS/MS

Sopan R. Marathe, Sonal S. Maheshwari, Shailesh S. Chalikwar and Atul A. Shirkhedkar*

ABSTRACT

Bioanalytical method development plays importance job in the preclinical and clinical studies. Pharmacokinetics of any medication and additionally its metabolite can be perceived by bioanalytical ponders. The quantitative examination of medications and their metabolites in the organic media is finished by bioanalytical studies. Physico-substance and organic techniques are utilized for these examinations. Each bioanalytical method should be particular, delicate and solid for the quantitative assessment in medication revelation process. Bioanalytical technique advancement comprises of sample preparation, chromatographic partition and detection by utilizing legitimate scientific strategy. Each developed technique ought to be approved according to the regulatory specialists, to give dependable and reproducible strategy for the expected use. Many analytical methods can be used for bioanalysis; LCMS/MS is one of them. In Liquid chromatography-mass spectrometry (LC-MS/MS) the partition of analyte is finished by LC and location is completed by MS. LC-MS/MS conspicuously utilized in assessment and interpretation of bioavailability, bioequivalence and pharmacokinetic information. This survey additionally engaged on various approval parameters, for example, accuracy, precision, affectability, selectivity, standard curve limits of quantification, range, recovery stability, etc.

Keywords: Bioanalytical Method development, LC-MS/MS, Bioavailability and Bioequivalence.