FRUIT EXTRACT OF VANILLA (Vanilla planifolia Andrews) LOWERS TOTAL BLOOD GLUCOSE IN ALLOXAN-INDUCED HYPERGLYCEMIC MICE

Mohammad Kanedi, Septika Nurhidayah*, Endang Nurcahyani and Endang L. Widiastuti

ABSTRACT

Hyperglycemia, a high level of blood glucose, is a main indicator of diabetes mellitus. Diabetic patients that do not manage the sugar levels in their blood can suffer from many severe complications. One of plant origin bioactive suspected to have anti-hyperglicemic activity is vanillin. This study aimed to determine whether ethanol fruit extract vanilla (Vanilla planifolia Andrews), the main source of vanillin, can be used as anti-hyperglicemia in mice induced by alloxan (150mg/kg). Male mice (n=24) were divided into six groups. Group 1 (normal control) did not receive induction or treatment. Group-2 (negative control) was induced with alloxan but received no drug. Group-3 (possitve control) was treated with glibenclamide at the dose of 0.039 mg/kg. Whereas group-4, 5 dan 6 were given vanilla extract of 2.1, 4.2, dan 8.4 mg/g body weight respectively. The treatment were given once daily for 14 days. Blood glucose levels were examined on day-0 (base line), 6th, 13th and 20th. The results showed by day-13 all levels of vanilla extract have significantly reduce blood glucose levels compared with negative control group. On the 20th day the effect of reducing blood sugar by vanilla fruit extract has even surpassed the glibenclamide. It can be concluded that ethanol fruit extract of vanilla is potential to be used as antidiabetes.

Keywords: Alloxan, Antihyperglicemia, Diabetes mellitus, Hyiperglicemia, vanillin, Vanilla planifolia.