PHYTOCHEMICAL DETERMINATION OF A POLYHERBAL EXTRACT USING FTIR AND GC-MS ANALYSIS

Monisha Sudhakar and Ragavan Balliah*

ABSTRACT

The present study was carried out to characterize the bioactive constituents present in the polyherbal extract using FTIR and GC-MS. From the crude extracts for GC-MS analysis, 10 g sample is extracted with 30 ml ethanol, filtered in ash less filter paper with 2 g sodium sulphate and the extract is concentrated to 1 ml by bubbling nitrogen into the solution. The compound detection employed the NIST Ver.2.0 Year 2005 library. The FTIR spectrum confirmed the presence of aromatic substituted alkaloid functional groups. The results of the GC-MS analysis provide different peaks determining the presence of 16phytochemical compounds. The major phyto constituents were 1H- pyrrolo (2,3c) pyridine-3-propanoic acid, 5(4H)oxo-6,7, dihydro (100%), pentadecanoic acid (100%),5-5'Biphthalide(100%). Hence, this study proves the various active phyto constituents present in the polyherbal crude extract.

Keywords: Polyherbal (PH) extract; GC-MS; FTIR.