ANTIOXIDANT ACTIVITIES OF THE TOTAL AQUEOUS EXTRACT OF PHYLLANTHUS MUELLERIANUS LEAVES IN DIABETIC RATS

Gnaléi Rabé Marcellin*, Bahi Calixte, GUI Prisca Angèle, Tiekpa Wawa Justine and Coulibaly Adama

ABSTRACT

Diabetes mellitus is accompanied by oxidative stress and free radical production by various mechanisms. This oxidative stress is involved in the aggravation of the disease as well as in the occurrence of chronic complications related to diabetes. The objective of the present work is to study the antioxidant activity of the total aqueous extract of Phyllanthus muellerianus in diabetic rats. Diabetes is induced by a single daily dose of 10 mg / kg bw streptozotocin (STZ) for twenty-one (21) days. The animals were then treated for seven (7) days with a dose of different concentrations of the total aqueous extract (ETAq) of Phyllanthus muellerianus leaves (100, 200 and 300 mg / kg bw) and Glucidoral® (Glu) (10 and 20 mg / kg bw), a standard antidiabetic agent. After treatment, these rats were sacrificed, the liver was removed for the determination of superoxide dismutase and catalase activities, and the concentration of malondialdehyde. The induction of diabetes decreased the activities of superoxide dismutase and catalase, and increased blood glucose and malondialdehyde concentration in the liver of diabetic rats. In contrast, treatment with the aqueous total extract (ETAq) of Phyllanthus muellerianus and Glucidoral® (Glu) increased the activities of superoxide dismutase and catalase, and decreased blood glucose and concentration of malondialdehyde in the liver of these animals. The results showed that the total aqueous extract (ETAq) of Phyllanthus muellerianus leaves can protect cells from the oxidative stress that accompanies diabetes.

Keywords: Phyllanthus muellerianus, antioxidant activity, glucidoral, diabetic rats.