EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

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An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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Abstract

STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF TOPIRAMATE USING RP-HPLC IN BULK AND PHARMACEUTICAL DOSAGE FORMS

*Ranjana Kumari, Shyamala and Dr. J. V. C. Sharma

ABSTRACT

The present project work is aimed on stability indicating method development and validation of topiramate using RP-HPLC in bulk and pharmaceutical dosage form . The method developed will be accurate, precise and linear for the estimation of the drugs in the near future. The analytical method will be developed by studying different parameters of Topiramate. The column used for study is Inertsil C18, ODS chosen good peak shape. Ambient temperature is found to be suitable for the nature of drug solution. The flow rate will be fixed at 1.0ml/min to obtain good peak area, satisfactory retention time and good resolution. Different ratios of mobile phase will be studied, mobile phase with satisfactory results will be selected for good symmetrical peaks and good resolution. We conduct the analysis based on UV detection and Reverse Phase High Performance Liquid Chromatograph.

Keywords: Topiramate, RP-HPLC, UV spectrometer.


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SJIF Impact Factor: 7.065

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