TETRA-PRIMER ARMS-PCR AS AN EFFICIENT ALTERNATIVE FOR SNPS DETECTION IN MOLECULAR DIAGNOSTIC: A COMPARISON STUDY

Sabah Linjawi*, PhD, Zuhoor Al-Gaithy, MBBCh, FRCS (I), Samar Sindi, MS, Norah Hamdi, MS, Ayman Linjawi, MD, SRCS (CA) and Aisha Alrofidi, PhD.

ABSTRACT

Objective: The aim of this research was to compare the efficiency of the tetra-primer amplification refractory mutation system–polymerase chain reaction (ARMS-PCR) with restriction enzyme for detection of SNP anomalies in breast cancer blood samples. Method: one hundred breast cancer patients were analyzed for SNP mutation using tetra-primer ARMS-PCR technique and restriction enzyme (Pst1), to identify allele distribution of LCN2 on exon one a SNP rs11556770 (G/T). In this case, a single nucleotide transforms the amino acid from Glutamine, Gln (CAG), to Histidine, His (CAT). Result: Both techniques, tetra-primer amplification refractory mutation system–polymerase chain reaction (ARMS–PCR) and restriction enzyme, yielded normal (non-pathogenic) genotype pattern for the same SNP of sets samples, wherein tetra-primer ARMS PCR was 444bp, 156b, While restriction enzyme (PstI) three band 261bp, 135bp, 48bp. Nevertheless, both results estimate a normal allele for both techniques. Conclusion: Tetra-primer arms PCR method attained high specificity and sensitivity, along with the fact that this technique is more affordable, it can therefore be concluded that this method is an efficient and convenient option for detection of SNP anomalies for diagnostic purposes.

Keywords: T-ARMS-PCR, breast cancer, LCN2, Pst1, SNP.