A REVIEW ON SUCCESSIVE GENERATIONS OF NATTOKINASE BASED FIBRINOLYTIC AGENT

Prabhu N.*, Gajendran T., Ravi Shankar K., Sandhiya Devi P., Srimathi K. and Rajapriya P.

ABSTRACT

Nattokinase (NK) is one such fibrinolytic enzyme with a wide range of applications in Pharmaceutical industry, health care and medicine etc. It provides health benefits like cure of hemorrhoids, Diabetes, Muscle spasms, poor healing, chronic inflammation, helps to improve blood clotting mechanism, improves blood circulation, blood viscosity etc. NK, a potent blood-clot dissolving protein used for the treatment of cardiovascular diseases, is produced by the bacterium Bacillus subtilis during the fermentation of soybeans to produce Natto. In this review we focused on screening of producing strains, Genetic Engineering and Fermentation Process optimization for microbial nattokinase production, extraction and purification of nattokinase.

Keywords: Nattokinase, blood clot dissolving protein, Bacillus substilis.