COLORIMETRIC METHOD DEVELOPMENT AND VALIDATION OF GABAPENTIN IN BULK AND SOLID DOSAGE FORM

Dr. G. Abirami* M. Kowsalya, S. Pavithra, S. Priyanga, V. Sasirekha and N. Amudha

ABSTRACT

A simple, accurate, precise and economic spectrophotometric method for the determination of gabapentin is described. The method is based on the formation of brown coloured chromogen with 1,2-naphthoquinone 4-sulphonic acid reagent by gabapentin in alkaline medium. The coloured species has an absorption maximum at 454 nm with 0.1 N sodium hydroxide. Beer's law obeys over the concentration range of 10-60 μg mL-1 with regression coefficient of 0.998. The limit of detection and quantification value are 3.145 and 9.531 μg mL-1 respectively. Accuracy and precision of the developed methods have been tested in addition recovery studies have been carried out in order to confirm their accuracy. The method successfully applied to the determination of gabapentin in pharmaceutical formulation.

Keywords: Gabapentin, 1,2- naphthoquinone 4-sulphonic acid reagent, Colorimetry, Pharmaceutical preparations.