MOLECULAR STUDIES ON SELENIUM NANOPARTICLES IN DIABETES

Sayadat S. Abd- EL Maged, Doaa M. Abd-EL Fattah and Aya A. El Sobky*

ABSTRACT

The fundamental characteristic of selenium nanoparticles have given it a great priority to the attention of scientists and researchers, particularly in their medical applications, selenium nanoparticles identified as an antioxidant and also to protect the body and get rid of the harmful damage of free molecules resulting from oxygen metabolism, A vital and important role where it has been proven in a laboratory that it shields the body from the danger from the risk of heart illness, diabetes and cancer tumors. Therefore, this study was concerned the possible protective effect of CTS-SeNPs and Actozone drug in Sprague Dawely male rats. The experiment was applied on fifty Sprague Dawely male rats (140-160 gm) were prepared for this experiment. They were brought from Laboratory Animal Farm of the Faculty of Veterinary Medicine, Zagazig University. Results revealed that CTS-SeNPs + Actozone group had the highest body weight than other groups. Diabetic group revealed the highest glucose, liver enzymes activities, lipid profile and G6PD values and group treated with CTS-SeNPs showed significant decrease in values compared to the diabetic group. Diabetic group was the lowest insulin reading, total protein, albumin, globulin, hepatic oxidant/antioxidant, Pdx1 and Ngn3 genes levels and group treated with CTS-SeNPs showed significant increase in values compared to the diabetic group. Histopathological examination of all liver & pancreas specimens diabetic treated with CTS_SeNPs + actozone group; liver & pancreas of rat showed almost normal histological picture except for mild vascular congestion. In conclusion the results of this investigation demonstrated that DM-induced severe biochemical and histopathological changes in the liver and pancreas and CTS-SeNPs have a protective effect and minimize the risk of diabetic complications.

Keywords: Diabetes Mellitus, CTS-SeNPs, Actozone, Streptozotocin.