SEQUENCING RELATIONSHIPS BETWEEN OF NINE METALLO-?-LACTAMASE (MBL)-PRODUCING CHRYSEOBACTERIUM INDOLOGENES STRAINS IN KOREA

Jong Hwa Yum and Man Kyu Huh*

ABSTRACT

The genus Chryseobacterium (previously Flavobacterium) can be mostly found in soil and water. Some species of this genus are found in some foodstuffs. We applied PCR to the detection of the metallo-beta-lactamase gene, blaIND, in clinically isolated 31 Chryseobacterium indologenes strains. This study used our previous data (16S rDNA of metallo-β-lactamase of C. indologenes) for further analysis of studies. Aligned nucleotides of 16S rDNA of metallo-β-lactamase were varied within C. indologenes varying from 746 bp in blaIND 10 to 877bp in blaIND 11. The probability of changing from C to T was 21.7 percent, higher than other substitution probabilities. Number of segregating sites of C. indologenes was 349 and nucleotide diversity (π) was 0.175. Under the neutral mutation hypothesis, the probability that Tajima test statistic (D) is positive (1.065) is large than 0.5. In the hierarchy of strains, base arrangement showed polymorphism. Significant differences were expressed in synonymous/replacement ratio of them at the 0.05% level. There was heterogeneity in the sequencing type of IND enzymes. The heterogeneity of these sequences for IND enzymes may cause differences in function. The molecular identification of C. indologenes together with sequence heterogeneity of other metallo-enzymes may help to design novel metallo-β-lactamase inhibitors of clinical relevance.

Keywords: Chryseobacterium indologenes, Metallo-?-lactamase, Nucleotide diversity.