STANDARDIZATION OF BILWADI AGADA THROUGH HPTLC

Sk. Hafiz Hasan*, Anjali W. G., Gazala Hussain and Sudipana Sarkar

ABSTRACT

The quality control standards for the Ayurvedic polyherbal medicinal preparation is the need for the day in view of commercialization. Despite availability of modern equipments and techniques only a few Ayurvedic drugs have been standardized. Bilwadi agada (BA), an herbal preparation; mentioned in the text of Ayurveda in the context of treatment of poisoning, is one of those. High-performance thin layer chromatography (HPTLC) is an instrumental techniques and an analytical tool for chromatographic information. In this present study an effort has been made to standardize BA through HPTLC. TLC photo documentation of BA showed 13, 12 and 11 spots under short UV, long UV and under white light after derivatization respectively. Densitometric scan at 254 nm revealed 3 high peaks corresponding to 3 different compounds in the ethanol extract, compounds, at 366 nm and 620 nm there were three high peaks. These physico-chemical constants, TLC photo documentation, the unique Rf values and densitogram obtained at different wavelengths can be used as fingerprint to identify BA.

Keywords: Bilwadi agada, Standardization, HPTLC, Pharmaceutical study.