ADVANCED TECHNICAL AND MATHEMATICAL ASSESSMENT OF PEAK PURITY AND IDENTITY IN SALTING- OUT THIN LAYER CHROMATOGRAPHY FOR DETERMINATION OF SOME ORAL HYPOGLYCEMIC DRUGS

Yahya Abduh Salim Mohamed*

ABSTRACT

Four advanced technical and mathematical methods for assessment of peak purity and identity in salting out thin layer chromatography (SOTLC) of some oral hypoglycemic drugs have been concluded. The concluded methods help in accurate assessment of coefficient of similarity between the spectra for each compound as a pure substance or exists in pharmaceutical preparations. The study extended to derive equations for calculating the overlapped peak area between two adjacent peaks when the resolution between 0.0 and 1.0 and helping in reducing measurement errors (manual and automatic integration of the integrators).

Keywords: Peak purity and identity; integration; resolution; overlapped area; salting out thin layer chromatography; oral hypoglycemic drugs.