PROCESSING OF BONE MARROW MONONUCLEAR STEM CELLS IN ACUTE SPINAL CORD INJURY

*Tulika Chandra MD., Dr. R.N.Srivastava MS., Dr. Saroj Chooramani Gopal Mch., S.Nishat Fatima Rizvi PhD Scholar., Devisha Agarwal MBBS Scholar

ABSTRACT

The present study was conducted to evaluate the concentration of MNCs and its CD 34 positive cell fraction in the infusion sample of the autologous bone marrow concentrate used in the SCI patients. Bone marrow was the first source of mononuclear stem cells for experimental and later for clinical use. For experimental use, it is still probably the best and most accessible. 44 patients with loss of motor and sensory functions due to injury between T6 and L3 skeletal levels were selected. Finding shows that middle age groups [Mean ± SD (Range) =32.70 ± 9.80] are more prone to the SCI. among all the mode of injuries, the direct fall from height is the most common one (57 %) and L1, L2 and T12 are three main levels of motor loss due to SCI. There was a significant positive correlation of the total volume of bone marrow aspirated with bone marrow derived mononuclear cells (p<0.01, r=0.92). The volume of bone marrow aspirated had a significant positive correlation with the number of bone marrow derived CD34+ cells (p<0.01, r=0.81). The total number of mononuclear cells had significant positive correlation with bone marrow derived CD34+ cells (p<0.01, r=0.78). No significant association was found between mononuclear cells and CD34+ cells count with age and sex of patients. We found that the number of mononuclear cells and CD34+ cells were higher if greater amount of volumes of bone marrow was aspirated hence improving the chances of recovery.

Keywords: SCI, CD34+, MNCs, BMC, BM.