EFFECT OF KIHADALACTONE ON PLASMA RECALCIFICATION TIME OF NAJA KATIENSIS AND BITIS ARIETANS VENOMS TREATED BOVINE PLASMA

*Abdullahi Balarabe Sallau, Yusuf Tauhid, Abdullahi AbdulMalik Salman, Hadiza Lawal Abdullahi, Mohammed A Ibrahim, Joyce J Kiplimo, Muhammad Lawal Buga

ABSTRACT

The effect of a limonoid (Kihadalactone) on recalcification time of bovine plasma treated with Naja katiensis and Bitis arietans venoms was investigated. The plasma recalcification time was found to be effectively reduced in a dose dependent pattern. In plasma incubated with N. katiensis venom, the highest inhibitory activity of 42.3 % at 1 mg/ml dose of kihadalactone was obtained, this was however found to decrease to a lower inhibition value of 27.3 % at 0.001 mg/ml dose.
Similarly, in plasma incubated with B. arietans venom, an inhibitory activity of 51.2 % at 1 mg/ml dose of kihadalacetone and a low activity of 33.6 % inhibition of the increase at 0.001 mg/ml were obtained. This study showed that kihadalactone, could effectively serve as an antidote for the reversal of increase in plasma recalcification time posed by both snake venoms.

Keywords: Bitis arietans, Naja katiensis, snake venom and plasma recalcification time.