A STUDY ON ANTIULCER ACTIVITY OF BENINCASA HISPIDA LEAVES IN ULCER INDUCED RAT

Mastanaiah Juturu*, Chakrapani Bestha, Madhavi Latha Chennuru, Ushasree C.

ABSTRACT

Aim: The main aim of the study was to investigate antiulcer activity of aqueous, ethanol leaf extract of Benincasa Hispida by using various ulcer induced agents in rats. Methods: The authenticated drug Benincasa hispida was dried in shade and powdered coarsely. Extraction was done according to standard procedure using analytical grade solvents. The coarse powder of the Benincasa hispida was Sox let extracted with the solvents with increasing order of polarity i.e. Ethanol (64.5-65.5oc), and distilled water. The extracts obtained were concentrated under reduced pressure. Qualitative chemical tests were conducted for ethanol and aqueous extract of benincasa hispida. To identify the various phytoconstituents. BH powder extracts or standard drug or control vehicle was administered 30min. prior to pyloric ligation. Mean ulcer score for each animal is expressed as ulcer index. Results: Anti ulcer activity of BH Leaves was confirmed by using different rat models (pylorous ligation model.) and different doses of aqueous and ethanol extracts i.e. 250 mg/kg and 500 mg/kg of bodyweights. 8 mg/kg body weight of lansoprazole (pylorus ligation model) treated group increased the pH of gastric fluid to slightly neutral and reduced the volume of gastric fluid when compared to controlgroup. aqueous and ethanol extracts treated groups at the dose level of 250 mg/kg and 500mg/kg body weight showed nearly same results of pH as that of control group i.e. acidic. The volume of gastric fluids in aqueous and ethanol extracts treated groups at the dose level of 500mg/kg body weight has decreasedsignificantly. Among all the treated groups, aqueous and ethanol extracts of BH Leaves at the dose level 500 mg/kg body weight offered greater percentage protection by reducing ulcer index in (pylorus ligation model) studied when compared with standards. Lansoprozole (pylorus ligation model) respective standardused. Conclusion: Flavonoid is an anti-oxidant and its mucosal barrier protecting capacity may be responsible for the anti ulcer activity of BHLeaves.

Keywords: Anti-oxidant, Anti-ulcer activity, Benincasa hispida, Flavanoid, Pylorus ligation model.