STUDY THE PROTECTIVE EFFICACY OF MIXED SOLVENT EXTRACT OF ANDROGRAPHIS PANICULATA NEES IN DIFFERENT PROPORTIONS AGAINST CHROMIUM (VI)-INDUCED TOXICITY

Durga Pada Dolai, Somenath Roy and Sankar Kumar Dey*

ABSTRACT

Potassium dichromate (K2Cr2O7) has been demonstrated to induce oxidative stress and carcinogenic in nature. In the present study, the protective effect of aqueous and methanol extract of Andrographis paniculata in different proportions was studied against Cr (VI) induced toxicity of liver and lung mitochondria of male albino rat. A group of male albino rats (80-100 g) were obtained and divided into six groups. The animals of five groups were induced by interperitonial injection with K2Cr2O7 at a dose of 0.8 mg per 100 g body weight per day (20% LD50) for a period of 28 days. The animals of four of the chromium treated groups serving as the supplemented groups were injected with mixed hydro-methanol extract in the ratio of 70:30, 60:40; 50:50; 40:60 at a dose of 500 mg/kg body weight daily at an interval of six hours after injection of K2Cr2O7 for a period of 28 days. The animals of the remaining group received only the vehicle (0.9% physiological saline), served as control. After completion of chromium-treatment the animals were sacrifice and intact liver and lungs were dissected out for further use. Measurement of lipid peroxidation (MDA), conjugated dienes and antioxidants were used to monitor the antiperoxidative effects of different solvent extract in liver and lungs mitochondria. The increased lipid peroxides, conjugated dienes and NO release in liver and lungs of chromium-treated rats was accompanied by a significant decrease in the levels of glutathione (GSH and GSSG) and the activities of glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione-S-transferase (G-S-T), superoxide dismutase (SOD) and catalase (CAT). The important findings in this study corroborated the facts that particular 60:40 mixed hydro-methanol solvent extract of Andrographis paniculata has greater potential benefit than other ratio of mixed hydro-methanol solvent extract like 70:30, 60:40, 50:50 and 40:60 in maintenance of oxidative equilibrium, scavenging of ROS and augmented anti-oxidant defense against chromium-induced toxicity in liver and lungs mitochondria.

Keywords: Chromium, Animal, Liver, Lungs, Toxicity, Oxidative stress, Andrographis paniculata Nees.