INVITRO CYTOTOXIC EFFECT AND APOPTOSIS INDUCTION OF HYDROALCOHOLIC EXTRACT OF BOERHAAVIA DIFFUSA LINN AGAINST HUMAN CANCER CELL LINES

*G. Nalini, N. Chidambaranathan, S. A. Syed Ibrahim and K. Marihrishnaa

ABSTRACT

The aim of the present study is to evaluate the effect of in-vitro anticancer activity of the Hydroalcoholic extract of Boerhaavia diffusa linn against MCF-7 breast cancer & HCT-116 colon cancer cell lines. The viability of cells were evaluated by direct observation of cells by Inverted phase contrast microscope and followed by MTT assay method. The percentage of cell viability was found to be 97% at 6.25μg/ml on HCT-116 which decrease to 28% with increase in concentration of extract at 100μg/ml. Similarly for MCF-7 cell line, cell viability shows 70% at a concentration of 6.25μg/ml that reduced to 40% at 100μg/ml. The effect of hydro-alcoholic extracts of Boerhaavia diffusa linn on cell morphology of MCF-7 and HCT-116 cells was examined using phase-contrast microscope. Treatment with plant extract showed typical morphological changes on MCF-7 & HCT-116 exhibited condensed chromatin, apoptotic bodies with membrane blebs and cell shrinkage. Apoptosis changes and chromatin condensation were also determined by fluorescent microscope. The results indicate that Boerhaavia diffusa is a considerable source of natural bioactive substances with antiproliferative activity on HCT-116 & MCF-7 cells & significant morphological effects.

Keywords: Antiproliferative, MTT assay, apoptosis, chromatin condensation, Boerhaavia diffusa linn.