NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF VILAZODONE IN PHARMACEUTICAL DOSAGE FORMS

N. Appala Raju*, J. Raghu Ram, V. Kiran Kumar and VVSS Appala Raju

ABSTRACT

A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Vilazodone in Tablets dosage form. A Inertsil ODS 3V C18, 250x4.6 mm i.d, 5 μm partical size, with mobile phase containing solvent-A as 0.1% Ammonium dihydrogen phosphate in water with pH adusted to 3.2 with ortho-phosphoric acid in 1000ml water and solvent-B as acetonitrile in gradient mode of separation was used. The flow rate was 1 ml/min and the effluents were monitored at 265 nm. The retention time was 4.1 min. The detector response was linear in the concentration of 20-240 mcg/ml. The respective linear regression equation being Y= 43515.779x+22939.55. The limit of detection and limit of quantification was 0.05 and 0.15 mcg/ml respectively. The percentage assay of Vilazodone was 99.16 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate; this is useful for the routine determination of Vilazodone in bulk drug and in its pharmaceutical dosage form.

Keywords: Vilazodone, RP-HPLC, Estimation, and Tablets.