METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF TELMISARTAN AND ATORVASTATIN BY RP-HPLC

Ramakrishna Reddy Voggu*, Ravi Teja. Tumburu, M. Kishore

ABSTRACT

The present work describes a simple, rapid, and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of Telmisartan and Atorvastatin. C18 column (Inertsil-Extend C18 (250 × 4.6 mm, packed with 5 μm) and a mobile phase containing Buffer: ACN, 40:60 v/v mixtures was used for the separation and quantification. The flow rate was0.8 mL/min and the eluents were detected by UV detector at 250 nm. The retention times were found to be 2.766 and 5.383 mins, respectively. The developed method was validated according to ICH guidelines Q2 (R1) and found to be linear within the range of 10-100 𝜇g/mL for both drugs. The developed method was applied successfully for assay of Telmisartan and Atorvastatin in their combined in-house developed dosage forms and in vitro dissolution studies.

Keywords: Telmisartan, in vitro, Atorvastatin, liquid chromatography (RP-HPLC).