PHYTOCHEMICAL INVESTIGATION AND CHROMATOGRAPHIC ANALYSIS OF ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA WILD

Mohamed Shiek Arabath S. A., Syed Ibrahim S. A.*, Sesha Kumar S. S., Packia Lakshmi M., Sundarapandian M.

ABSTRACT

Chromatography techniques such as HPTLC and HPLC are commonly used to produce a fingerprint of Phytoconstituents. It allows identification and quantification of the main constituents within the plant. The aim of this study is to investigate the phytochemical constituents and comparing the HPTLC and HPLC quantification analysis of berberine in ethanolic extract of stem part of Tinospora cordifolia (ESTC). The HPTLC is effective for qualitative analysis, whereas HPLC is more accurate for quantitative analysis. A combination of these two methods may be useful in a quality control Testing as it would allow rapid qualitative analysis of herbal material while maintaining accurate quantification of extracts.

Keywords: Tinospora cordifolia linn, HPTLC, HPLC, berberine, quantification and phytochemical constituents, T.cordifolia.