DESIGN AND SYNTHESIS OF BIOGENIC SILVER NANOPARTICLES FROM AQUEOUS LEAF EXTRACT OF MIRABLIS JALAPA L., AND EXPLORING IT’S INVITRO ANTI-CANCER ACTIVITY

M. Sangeetha* and C. Mahendran

ABSTRACT

In recent science Nanotechnology is a burning field for the researchers. Nanotechnology deals with the Nanoparticles having a size of 1 – 100 nm in one dimension used significantly concerning medicinal chemistry, atomic physics, and all other known fields. Mirablis Jalapa L(Family: Nyctaginaceae) is versatile medicinal plants enriched with novel bioactive molecules and displays board-spectrum pharmacological actions including anticancer potential. The study planned to biosynthesize anticancer potent silver nanoparticles using aqueous leaf extract of Mirablis Jalapa. The synthesized nanoparticles were confirmed by colour transformation and UV-Vis spectrophotometry. The size and morphology of the silver nanoparticle were characterized by Scanning electron microscope (SEM). The stability of silver nanoparticles were detected by Fourier Transform Infra Red Spectroscopy (FT-IR). EDAX and Zeta potential were also detected for silver nanoparticles. Anticancer activity of silver nanoparticles tested against Dalton’s Lymphoma Ascites (DLA) and Ehrlich Ascites Carcinoma(EAC) cells. The appearance of reddish brown colour and UV absorption 428 nm (OD=0.0695) confirmed the synthesized silver nanoparticles. The silver nanoparticles showed spherical structure and their size were ranging from 1- 100 nm under SEM observations. FT-IR spctra of silver nanoparticles showed the peaks for the functional groups, C=O,-C=C,C-H and OH which indicate the stability of synthesized silver nanoparticles. The synthesized of silver nanoparticles using aqueous extract of leaves of Mirablis Jalapa would be helpful for the preparation of potent cytotoxic agents against DLA and EAC cells to destroy cancer cells.

Keywords: Mirablis Jalapa, Green synthesis, Silver nano particles, EDAX, Anti cancer activity.