IN VITRO ANTIMICROBIAL SCREENING OF MULTIDRUG RESISTANT BACTERIA BY ISOLATED FRACTION FROM HETEROPHRAGMA ADENOPHYLLUM LEAVES

Vilas Surana*, Dinesh R. Shah and Sri Hari Mishra

ABSTRACT

The present study aimed to screen the in vitro antimicrobial activity of ethyl acetate fraction isolated from crude chloroform extract of the leaves of Heterophragma adenophyllum against Multidrug-resistant pathogenic bacteria such as Escherichia coli, Salmonella enterica, Staphylococcus aureus, Bacillus anthracis and Klebsiella pneumonia broad spectrum microorganism. The study was carried out by using standard nutrient broth for microbes and streptomycin was taken as a positive control. Ethyl acetate fraction used as a test sample and DMSO as a negative control. From the results of the zone of inhibition, it was concluded that the ethyl acetate fraction from crude chloroform extract was pusses in vitro antimicrobial activity while results of minimum inhibitory concentration, Plant-derived, and other safe natural antimicrobial compounds have the potential to control the prevalence of both susceptible and resistant pathogens in various environments it was revealed that all bacterial strains were sensitive towards selected fraction from the plant for the study.

Keywords: Antimicrobial, multidrug, Heterophragma adenophyllum, ethyl acetate fraction.