EUROPEAN JOURNAL OF PHARMACEUTICAL AND MEDICAL RESEARCH CHEMICAL CONSTITUENTS FROM THE LEAVES OF CALLISTEMON LANCEOLATUS (SM.) SWEET

Shahnaz Sultana, Mohammed Ali* and Showkat Rassol Mir

ABSTRACT

Callistemon lanceolatus (Sm.) Sweet (Myrtaceae) grows all over the world and is used as a tea substitute with a delightfully refreshing flavor and to treat bronchitis and cough. This research work was undertaken to characterize structures of chemical constituents isolated from the plant leaves. A methanol extract of the leaves was adsorbed on silica gel (60-120 mesh) and subjected to silica gel column packed in petroleum ether. The column was eluted with petroleum ether, chloroform and methanol, successively, in order of increasing polarity to isolate the compounds. The isolated chemical constituents were characterized as 4-methoxyphenyl 4′-octanyl ether (1), 5,7-dihydroxy-4′-methoxy-8-ethyl flavone (8-ethyl 4′-methoxyapigenin, 2), 5,7,4′-trimethoxy-6-methyl-8-isopropanolyl apigenin (3), lanostan-3β,19-, 18,21-diolide (callistelanostanediolide, 6), 4′-methoxy-8-(2′′-propanolyl) apigenin (7), 4-hydroxyphenyl propanoloxycerotate (8), 4-hydroxyphenethyl tetratriacontanoate (tyrosolylgheddate, 9), 4-hydroxyphenyl 1-propanyl tetratriacontanoate (4-hydroxyphenyl propanyloxygheddate, 10) along with the known constituents 1-heptacosanol (4), 1-octacosanol (5) and α-amyrin (11). Their structures were established on the basis of spectral data analysis and chemical reactions.

Keywords: Callistemon lanceolatus, leaves, extraction, phytoconstituents, isolation, spectral data, characterization.