ANTIDIABETIC EFFECTS OF MORINGA OLEIFERA ON PANCREATIC ISLET CELL OF ADULT MALE WISTAR RATS

Memudu Adejoke Elizabeth, Ewaoche Elizabeth Jenebu, *Odetola Amos Amoo and Adeleye Olufunto Omodele

ABSTRACT

Background: Diabetes mellitus is a complex metabolic disorder, which is life threatening as a result of an insufficient action of insulin or pancreatic β-cell dysfunction. This study was carried out to evaluate anti-diabetic effects of aqueous extract of Moringa seed on pancreatic islet cell in alloxan induced diabetic male Wistar rats. Materials and methods: Twenty- five (25) Adult Male Wistar rats were divided into five (5) groups (n=5). Administration was done via oral route over a period of two weeks. Group A: Control= normal saline, Group B diabetic model (120mg/kg of alloxan), Group C: 100mg/kg body weight of aqueous extract of Moringa Oleifera seed, Group D:diabetic model treated with 100mg/kg of aqueous extract of Moringa Oleifera seed and Group E: diabetic model treated with standard diabetic drug (5mg/kg Glibenclamide). Serum was analysed for lipid peroxidation enzyme-Malondialdehyde (MDA) and antioxidant enzymes Superoxide dismutase (SOD) activities while pancreas was processed for Haematoxylin and Eosin stain and Periodic Acid Schiffs stain for glycogen. Results: The results showed a significant decrease in final body weights of the experimental animals (P<0.05) compared to the control and their respectives initial body weights. Lipid peroxidation increased in group B as compared to A, C D and E. There was no significant difference in Mo and GLB reduction in peroxidation activity MDA as well as SOD levels. There was no distortion of the clustered Langerhans cells in the control and MO, GBL treated groups but severe distruption in Alloxan treated group. Conclusions: Moringa Oleifera has a potential cytoprotective effect on Islet of Langerhans against oxidative stress mediated diabetic disorder and can be used as a therapeutic in diabetic disease prevention and management.

Keywords: Moringa Oleifera, Diabetic, Langerhans Cells, Glibenclamide, Malondialdehyde and Superoxide Dismutase.