SCREENING AND BIOSYNTHESIS OF L-ASPARAGINASE FROM FUSARIUM SP THROUGH SUBMERGED FERMENTATION

Divyashree K. C., Nagalambika Prasad, Siddalingeshwara K. G.*, Prakruthi G. and Roopa B.

ABSTRACT

L-asparaginase production using microbial systems has attracted considerable attention due to its cost-effective and eco-friendly nature. Although the enzyme has been found in a variety of bacteria, fungi and actinomycetes and mammals, few of the purified preparations have shown to possess anti-tumor or anti-leukemic activity. Ten Fusarium sp were isolated from soil samples from different regions in Bangalore. Isolated fungal strains were screened for l-asparaginase production by plate assay. Fusarium sp D 05 were selected as best L-asparaginase producer (1.03 cm) and were confirmation carried out by Thin layer chromatography (Rf value is 0.80). The Fusarium sp D 05 were employed for further fermentation kinetics studies. The different pH (6), temperature (350C) and inoculum size (1 ml) were optimized and it showed 119.7 IU of L-asparaginase activity. Further studies were carried out by Extraction and partial purification of L-asparaginase by salt precipitation (ammonium sulphate) by 80%. The partially purified enzyme were used for characterization of L-asparaginase and antitumour properties study.

Keywords: Fusarium sp, L-asparaginase, Plate assay and submerged fermentation.