AN APPROACH ON MICROBIAL PRODUCTION OF LACCASE FROM FUSARIUM SP

Pallavi S., Archer Ann Catherine, Dr. Siddalingeshwara K. G.*, Prakruthi G. and Roopa B.

ABSTRACT

Laccases are most stable and powerful biocatalysts with broad applications. It is a copper containing, organic solvents resistance, oxidoreductase enzyme, having potential ability to oxidize the phenolic and non-phenolic compounds. Laccases play an important role in food industry, paper and pulp industry, textile industry, synthetic chemistry, cosmetics, soil bioremediation and biodegradation of environmental phenolic pollutant. Fifteen Fusarium sp were isolated from different soil samples from different locations in Bangalore. Isolated fungal strains were screened for Laccase production by plate assay by different substrate using ABTS (2-2’-Azino-bis-[3-ethyl benzthiazoline-6-sulfonic acid]), guaiacol, and tannic acid as a substrates and the culture plates were observed for color change in the media. Fusarium sp P 15 were selected as best laccase producer. The Fusarium sp P 15 were employed for further production of Laccase studies. The different pH (6), temperature (350C) and inoculum size (1.0ml) were optimized and it showed 1.05 IU of Laccase activity.

Keywords: Laccase, Submerged fermentation, ABTS, guaiacol, and tannic acid and Fusarium sp.