PRODUCTION AND CHARACTERIZATION OF NDV IGY FROM TWO DIFFERENT CHICKEN BREEDS

Salwa Mohamed Hezema, Samy Kasem, Emad Eldin Elgendy, Madiha Salah Ibrahim*

ABSTRACT

This study aimed to prepare hyper-immune chicken IgY against Newcastle disease virus (NDV) and studying its immunogenicity against field isolates of NDV. Ten white Hy-line layers, and ten local house breeds were used. The birds received an initial immunization followed by two booster doses at time intervals of 2 weeks. Throughout the experiment (8-11) weeks, serum samples were collected weekly with daily egg collection. The purified IgY specificity and titers in sera and egg yolk were determined using HI and neutralization tests against NDV vaccinal strain and field isolates. Significant titers of IgY were detected against NDV with comparable neutralization efficacy for the two breeds. Thus, both hen breeds can be efficiently used for production of protective IgY against NDV field isolates.

Keywords: NDV, IgY, HI, Neutralization test.